When selecting centrifuge tubes, it’s important to consider the material, sealing mechanism, clarity, and chemical resistance needed for your application. This guide covers tips for choosing the right tubes and rotor types as well as best practices for cleaning, storage and centrifugation. Properly caring for tubes ensures sample integrity, prevents leakage or breakage, and prolongs tube life.

Key Factors in Selecting Tubes

1. Assess suggested sample volume and max speed using product guidelines or package labels

2. Match tubes carefully to rotor type to prevent breakage and sample loss

3. Consider chemical resistance based on material: PP and PA are opaque with good resistance; PC and PET are clear with poor resistance

Popular Rotor Types and Recommended Tube Orientation

• Swinging bucket rotors: Tubes hang vertically then swing out horizontally during rotation
• Fixed angle rotors: Tubes remain fixed at an angle during rotation
• Vertical rotors: Tubes stay vertical throughout rotation
• Fill thin-walled sealed tubes completely for fixed angle or vertical rotors

Proper Cleaning and Sterilization of Tubes

• Clean with mild detergent, warm water, rinse and air dry
• Avoid glassware washers or dishwashers due to excess heat
• Only autoclave if necessary, at 121°C for 15 minutes
• Prevent sample loss by matching tubes carefully to rotor

Safe Centrifugation Practices

Always balance tubes properly in centrifuge. Never exceed max speed rating for tubes. Stop rotation immediately if an imbalance is suspected. Avoid overfilling tubes beyond stated capacity

Proper Storage for Optimal Tube Lifespan

Store tubes with caps loosened in a cool, dry location. Ensure tubes are fully dried after washing before capping for storage. Regularly inspect tubes for cracks/wear and replace as needed. Follow manufacturer date recommendations for tube expiration/replacement

Signs Your Tubes May Need Replacing

Fogging, crazing or discoloration of plastic. Difficulty closing or opening caps properly. Leaks at cap seam or bottom seal. Cracks along tube body or cap

Conclusion

By selecting the optimal centrifuge tubes for your application and properly caring for them during use, cleaning and storage, you can ensure reliable performance and lifespan. Always adhere to safety best practices during centrifugation to prevent tube breakage and sample loss. Reference manufacturer guidelines and replace tubes at first signs of wear.