Contamination can significantly impact the accuracy and reliability of your laboratory results. Here are some key steps to prevent it using pipette tips:  

General Prevention Measures

l Use a new tip for each sample: This is the most fundamental way to prevent cross-contamination between samples.

l Avoid touching the tip with your fingers: Your hands are a major source of contamination. Wear gloves and avoid touching the tip's exterior.  

l Use filtered tips: These tips contain a porous filter that prevents aerosols and liquid droplets from entering the pipette, reducing the risk of contamination.  

l Proper pipetting technique: Ensure you are using the correct pipetting technique to avoid creating aerosols or drawing liquid into the pipette body.

l Regularly clean the pipette: Follow the manufacturer's instructions for cleaning and disinfecting your pipette to prevent buildup of contaminants.

Specific Contamination Types

Sample-to-sample contamination

l Use filtered tips.

l Change tips between samples.

l Consider using aerosol-resistant tips.

Sample-to-pipette contamination

l Hold the pipette vertically during pipetting to prevent liquid from entering the pipette body.  

l Use filter tips.

l Avoid immersing the tip too deeply into the liquid.

l By following these guidelines, you can significantly reduce the risk of contamination and ensure the accuracy of your experiments.